English translation for "hybridization in situ"
|
- 原位杂交
Related Translations:
- Example Sentences:
| 1. | Studies on detection of nosema bombycis by hybridization in situ 家蚕微孢子虫原位杂交诊断技术研究 | | 2. | Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned 使用sau3ai对基因组dna进行不完全酶切,回收2 3kb片段,与puc18质粒连接转化大肠杆菌,利用地高辛标记探针,使用菌落原位杂交筛选转化子;筛选到包含有约1kb外源片段的转化子。 | | 3. | In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks 方法:胰岛的分离参照王玲等的方法,每次实验取新生3 - 5天sd大鼠,无菌条件下剖腹取出胰腺,剪切为0 . 5mm ~ 3的组织块, v型胶原酶消化30min后,离心洗涤,悬浮于完全培养基,接种入50ml培养瓶,于5 co _ 2 、 95空气条件下培养20h ,转板纯化,接种于96孔培养板培养24h ,按实验要求进行实验。 |
- Similar Words:
- "hybridization arrest experiment" English translation, "hybridization arrest of translation" English translation, "hybridization by means of mixed sperms" English translation, "hybridization effect" English translation, "hybridization histochemistry" English translation, "hybridization of atomic orbitals" English translation, "hybridization of nucleic acid" English translation, "hybridization of olap" English translation, "hybridization oven" English translation, "hybridization probe" English translation
|
|
|